The purpose of the present invention is to provide a method for the expansion culture of nephron progenitor cells while maintaining an undifferentiated state. Another purpose of the present invention is to provide an expansion culture method for cultured nephron progenitor cells to have nephron-forming ability. Yet another purpose of the present invention is to provide an expansion culture method for nephron progenitor cells that makes it possible to achieve an excellent expansion rate. The present invention provides a method for culturing and increasing nephron progenitor cells created from isolated mammalian nephron progenitor cells or iPS cells using a medium containing the following compounds: (i) Wnt agonist; (ii) Notch inhibitor; (iii) Bmp; (iv) leukemia inhibitory factor; (v) Rock inhibitor; and (vi) Fgf.
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