Effect of the Surface Density of Nanosegments Immobilized on Culture Dishes on Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood

摘要

Hematopoietic stem and progenitor cells (HSCs) are multipotent cells that have the specific capacity to self-renew and differentiate into all type of mature blood cells. UCB is an attractive source of HSCs because there is lower risk of graft-versus-host disease (GVHD) for UCB transplantation compared to bone marrow or peripheral blood transplantation. However, the low number of HSCs and small volume (50-150 mL) obtainable from a single donor of UCB limits direct transplantation of UCB to the treatment of pediatric patients. A density of approximately 1.7x10~7 CD34~+ cells/kg is necessary for the transplantation of UCB into patients. Therefore, UCB transplantation has been limited to children with an average weight of 20 kg.~(1-3)