Peripheral Blood Hematopoietic Progenitor/Stem Cells Proliferate to Form Coloniesin Liquid Culture but Require Contact with Vascular Endothelial Cells and GM-CSF

摘要

To test the hypothesis whether peripheral blood hematopoietic progenitor/stemcells (PBSCS) interact with vascular endothelial cells during events leading to extramedullary hematopoiesis, we cocultured T-cell depleted, peripheral blood mononuclear cells obtained from cytokine treated primates in liquid culture containing a monolayer of porcine aortic endothelial cells (PAECs) for 7 days. Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) added to cocultures of PBSC-PAEC stimulated colony formation, while only a few clusters were observed in cultures without GM-CSF. In contrast, colony formation was not stimulated when either interleukin 1 (IL-1) or IL-3 were added to the cultures.