hybrid molecule formula gm-csf - l - epo or epo - l - gm-csf for erythropoietic stimulation

摘要

PCT No. PCT/EP95/02011 Sec. 371 Date Nov. 27, 1996 Sec. 102(e) Date Nov. 27, 1996 PCT Filed May 26, 1995 PCT Pub. No. WO95/33057 PCT Pub. Date Dec. 7, 1995It is described a hybrid molecule of formula GM-CSF-L-EPO or EPO-L-GM-CSF useful for the stimulation of hematopoiesis, comprising GM-CSF and EPO molecules fused together by means of a linker L having a variable length of amino acids, GM-CSF and EPO molecules being either complete molecules or fragments thereof. Such hybrid molecules exhibit a higher specifity of action on erythroid differentiation if compared to that of an equimolar mixture of not-linked GM-CSF and EPO molecules. Further, the present invention describes: DNA sequences encoding such hybrid molecules, which stimolate the hematopoiesis; plasmid vectors containing the genes encoding hybrid molecules and directing their synthesis in host cells; the use of such hybrid molecules for the preparation of a pharmaceutical composition and such pharmaceutical composition useful for stimolation of hematopoiesis.