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System and method for automated nucleic acid amplification

机译:自动核酸扩增的系统和方法

摘要

An embodiment of a device for automatically executing a process of generating an emulsion containing nucleic acids, amplifying the nucleic acids in the emulsion, breaking the emulsion, and separating and purifying said amplified nucleic acids, is described that comprises an emulsion generation unit for sealing beads to which nucleic acids are bound in a water-in-oil type emulsion; a nucleic acid amplification unit provided with a reaction vessel for amplifying said nucleic acids and a heating and cooling part for heating and cooling the reaction vessel; an emulsion breaking unit for breaking the emulsion after nucleic acid amplification; and a nucleic acid purification unit for recovering said amplified nucleic acids from said emulsion breaking unit.
机译:描述了一种用于自动执行产生包含核酸的乳液,扩增乳液中的核酸,破坏乳液以及分离和纯化所述扩增的核酸的过程的装置的实施方式,该装置包括用于密封珠的乳液产生单元。油包水型乳剂中结合有核酸的核酸;核酸扩增单元,其具有用于扩增所述核酸的反应容器和用于加热和冷却反应容器的加热和冷却部分;破乳单元,用于在核酸扩增后破乳。核酸纯化单元,其用于从所述破乳单元中回收所述扩增的核酸。

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