Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator

摘要

"Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 39 complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target, at the same time, methods for producing a digital printing gDNA DNA and RNA of a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target nucleic acid, and use of the initiator.The present invention relates to an annealing control primer annealing to improve the specificity of the amplification of nucleic acid and its applications in all fields of tecnoloGIA involved in nucleic acid amplification.This initiator comprises (a) a portion of the extremity 3 39 having a sequ00b3u00eancia nucleotide hibridizadora substantially complementary to a site in a nucleic acid standard for hibridizThe air with the same; (b) a portion of the tip 5 39 having a nucleotide sequ00b3u00eancia arbitrary pre selected; and (c) a regulating portion positioned between said portion of the extremity 3 and 39That portion of the extremity 5 39 comprising at least a universal basis or analogue non discriminatory basis.By means of which the said portion is capable of regulating a regulating portion of the annealing of said initiator in combination with the annealing temperature.