Automated Sample Preparation (ASP): Development of a Rapid Method to Sequentially Isolate Nucleic Acids and Protein from Any Sample Type by a Cartridge-Based System.

摘要

CUBRC has developed an in-line, multi-analyte isolation technology that utilizes solid phase extraction chemistries to purify and concentrate nucleic acids and protein for downstream analysis. This technology will be automated by developing a single use, cartridge-based unit. Development of such a unit is critical to reaching the US Government's and Department of Defense's biological threat countermeasure development goals. Specifically, CUBRC will design and manufacture a prototype cartridge(s) and test the prototype cartridge for its ability to isolate each analyte individually and in succession. Testing will be performed on both laboratory derived samples and samples typical of those generated by aerosol collectors.