首页> 外文OA文献 >Degradation of remazol black B by bacillus cereus strain A from textile wastewater
【2h】

Degradation of remazol black B by bacillus cereus strain A from textile wastewater

机译:蜡样芽孢杆菌A降解纺织废水中的甲酚黑B

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Five bacterial strains designated as A, B1, B2, C and Y1 that were revived from the UTM glycerol stock were used for biodegradation study of azo dyes (SF BLACK EXA, SF RED 3BS, SFN BLUE 150 % and SF YELLOW EXF) at three different conditions; aerobic, facultative anaerobic and partial aerobic (without agitation). Screening test shows bacterium A, under facultative anaerobic condition demonstrated the highest decolourisation rate with removal rate (7.0% h-1) of 0.1 gL-1 SF BLACK EXA was observed. Relationship of growth and decolourization analysis shows decolourisation rate of the same azo dye by bacterium A in CDM medium under facultative anaerobic and aerobic conditions were 26.445% h-1 and 24.56% h-1, respectively. The growth rate of bacterium A was found higher in aerobic culture (2.733 mgh-1) in comparison with that in facultative anaerobic culture (1.067 mgh-1). In other assessment, bacterium A was applied into optimized CDM medium (glucose (1 gL-1), NH4Cl (0.5 gL-1), K2HPO4 (7 gL-1), KH2PO4 (2 gL-1), MgSO4.7H2O (0.1 gL-1), CaCl2 (0.02 gL-1), RBB (0.1 gL-1)), pH 6.5, incubated at 35 °C, with 10 % v/v inoculum under facultative anaerobic condition. Result showed that bacteria A successfully degraded more than 80 % RBB after 20 h incubation. Degradation of RBB molecules increased up to 90 % and 30 % COD removal before attaining stable condition within 164 h incubation. Azoreductase analysis revealed that enzyme was produced intracellularly (11.45 x 10-3 Umin-1) and stabled at pH 6-8 and temperature ranged from 30 ºC to 40 ºC. HPLC analysis revealed that biodegradation of RBB under combined facultative anaerobic-aerobic condition produced sulfanilic acid (Rt = 3.988 min) after 160 h incubation and two unknown metabolites (Rt = 6.071 min and 7.480 min) after 164 h incubations. The partial 16S rRNA analysis showed that bacterium A closely related to Bacillus cereus (99 % sequence similarity). These findings suggest that this bacterium has the capability to assist in the degradation of wastewater containing azo dyes from industry of textile-based.
机译:从UTM甘油库存中回收的五种分别命名为A,B1,B2,C和Y1的细菌菌株用于偶氮染料的生物降解研究(SF BLACK EXA,SF RED 3BS,SFN BLUE 150%和SF YELLOW EXF)。不同的条件;有氧,兼性厌氧和部分有氧(无搅拌)。筛选测试显示,在兼性厌氧条件下,细菌A表现出最高的脱色率,去除率(7.0%h-1)为0.1 gL-1 SF BLACK EXA。生长与脱色分析的关系表明,在兼性厌氧和好氧条件下,细菌A在CDM培养基中相同偶氮染料的脱色率分别为26.445%h-1和24.56%h-1。有氧培养(2.733 mgh-1)比兼性厌氧培养(1.067 mgh-1)的细菌A的生长速率更高。在其他评估中,将细菌A应用于优化的CDM培养基(葡萄糖(1 gL-1),NH4Cl(0.5 gL-1),K2HPO4(7 gL-1),KH2PO4(2 gL-1),MgSO4.7H2O(0.1 (gL-1),CaCl2(0.02 gL-1),RBB(0.1 gL-1)),pH 6.5,在兼性厌氧条件下于35°C与10%v / v接种液孵育。结果表明,细菌A在孵育20小时后成功降解了80%以上的RBB。在培养164小时内达到稳定状态之前,RBB分子的降解增加了高达90%和30%的COD去除率。固氮还原酶分析表明,酶是在细胞内产生的(11.45 x 10-3 Umin-1),在pH 6-8和温度范围从30ºC到40ºC时稳定。 HPLC分析表明,在兼性厌氧-好氧条件下,RBB的生物降解在160 h孵育后产生了磺胺酸(Rt = 3.988 min),在164 h孵育后产生了两种未知的代谢产物(Rt = 6.071 min和7.480 min)。 16S rRNA的部分分析表明,细菌A与蜡状芽孢杆菌密切相关(99%的序列相似性)。这些发现表明该细菌具有协助降解基于纺织工业的含偶氮染料的废水的能力。

著录项

  • 作者

    Ari Zainab;

  • 作者单位
  • 年度 2013
  • 总页数
  • 原文格式 PDF
  • 正文语种 en
  • 中图分类

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号