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Standardization of the bio-active compounds (rotenoids) from the extract of local plant species (derris elliptica) using the internal standard method of high performance liquid chromatography (HPLC)

机译:使用高效液相色谱法(HPLC)的内标方法对本地植物物种(椭圆草)提取物中的生物活性化合物(类胡萝卜素)进行标准化

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摘要

It is well known now that some plant species represent an efficient factory of chemicals, which are manufactured and used as bio-weapons against pest attacks. Extensive work has been done during the last few decades on these potentially useful compounds. During the last few decades a growing interest has been paid for safe agricultural production i.e free residual toxicity hazards to human beings and to the environment. Plant extracts-based biocides possess a great advantage compared with the chemical ones. Their efficacies are also acceptable. Research carried out was to standardize and determine the bio-active compounds from the extract of local plant species (Derris elliptica) using the internal standard method of the isocratic High Performance Liquid Chromatography (HPLC) analysis system. The raw plants were collected from Kota Johor Lama, Johor and sorted to collect the root and stem. Only the root and stem were utilized as a raw material of the extraction process. The root and stem were extracted using the Normal Soaking Extraction (NSE) process at 28 °C to 30 °C with 95.0 % (v/v) of Acetone as a solvent and the solvent-to-solid ratio of the extraction is (10.0 ml/g). The extraction was carried out for 24 hours and further cleaned up to remove fine debris of root and stem prior to determination of the rotenone and its derivatives content. The rotenone cube resin of SAPHYR S.A.R.L (France) was used to verify the appearances of the compounds in the extract. The employed method of analysis shows significant appearances of the bio-active compounds in the extract compared with the commercial grade of rotenone cube resin.
机译:现在众所周知,某些植物物种代表了高效的化学工厂,这些化学工厂被制造出来并用作抵抗有害生物侵袭的生物武器。在过去的几十年中,已经对这些潜在有用的化合物进行了广泛的研究。在过去的几十年中,人们越来越关注安全的农业生产,即对人类和环境的无残留毒性危害。与化学农药相比,基于植物提取物的杀菌剂具有很大的优势。它们的功效也是可以接受的。使用等度高效液相色谱(HPLC)分析系统的内标方法,对来自本地植物物种(Derris elliptica)提取物中的生物活性化合物进行标准化和确定。原始植物是从柔佛州的哥打柔佛州喇嘛(Kota Johor Lama)收集的,并进行分选以收集根和茎。仅根和茎被用作提取过程的原料。使用普通浸泡萃取(NSE)工艺在28°C至30°C下以95.0%(v / v)丙酮作为溶剂萃取根和茎,萃取的溶剂与固体比为(10.0 ml / g)。提取进行24小时,然后进一步清洗以除去根和茎的细碎屑,然后再测定鱼藤酮及其衍生物含量。 SAPHYR S.A.R.L(法国)的鱼藤酮立方体树脂用于验证提取物中化合物的外观。与鱼藤酮立方体树脂的商业等级相比,所采用的分析方法显示出提取物中生物活性化合物的明显外观。

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