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第二届环渤海色谱学术报告会

召开年：2012
召开地：青岛
出版时间： 2012-09-19

主办单位：山东化学化工学会;山东省理化分析测试协会;青岛市分析测试学会

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1.RP-HPLC法研究一清颗粒中5种有效成分的含量
- 李炳龙
- 《第二届环渤海色谱学术报告会》 | 2012年
摘要：本文采用反相高效液相色谱法测定一清颗粒中大黄酸、大黄酚、大黄素甲醚、盐酸小粟碱和黄芩苷的含量，方法简便、快速、准确，为该药品的质量控制提供了全面的、客观的定性定量评价方法，可作为控制一清颗粒质量的一种有效手段。
2.UHPLC-MS/MS法测定猴血浆中艾塞那肽浓度
- 张金凤;Jinfeng Zhang;Chunjie Sha;沙春洁;Yu Sun;孙瑜;Jiye Sun;孙吉叶;Yunyun Gai;盖芸芸;Jiangbin Han;韩江斌;Xin Shao;邵馨;Chunna Sha;沙春娜;李又欣;Youxin Li;Wanhui Liu;刘万卉
- 《第二届环渤海色谱学术报告会》 | 2012年
摘要：A highly sensitive ultra-high-performance liquid chromatographic-tandem mass spectrometry method (UHPLC/MS/MS) was developed for the quantification of the synthetic peptide drug of exenatide in monkey plasma. Sample preparation was carried out by solid-phase extraction (SPE)，and bivalirudin was used as the internal standard (I. S.).An excellent chromatographic separation was obtained on a reversed-phase C18 column with a gradient elution. Detection utilized a Qtrap 5500 μL system operated in the positive ion mode with multiple reaction monitoring (MRM).The proposed method was validated by assessing the specificity, linearity, intra-and inter-day precision and accuracy, recovery, and stability. The method resulted in a linear calibration range of 0. 10-30 ng/mL, extracting with only 50 monkey plasma aliquots. The intra- and inter-day precision (as relative standard deviation)were less than 7.5% and 9.6%, respectively. The method was validated and could be successfully utilized for the pharmacokinetic study of exenatide in monkeys following a single subcutaneous injection of Byetta.
3.高效液相色谱法测定人血浆中阿昔莫司浓度及其药代动力学研究
- 李荣;黄婧;卜凡龙;魏春敏;王本杰;郭瑞臣
- 《第二届环渤海色谱学术报告会》 | 2012年
摘要：目的：建立人血浆阿昔莫司的定量分析方法,为药代动力学研究提供技术手段。方法：采用高效液相色谱法,以Inertsil(g)/WondaSilTMC18(4.6×250mm,5μm)色谱柱为固定相；以乙腈∶2mM四丁基溴化铵(磷酸调pH值至3)=13∶87 (V/V)为流动相,咖啡因为内标。流速1.0mL/min检测波长263nm。结果：阿昔莫司在0.05～6μg/mL浓度范围内线性关系良好,标准曲线方程为y=0.74190342x-0.0025535 (r2=0.99926),最低定量限为0.05μg/mL,绝对回收率、精密度与准确度、稳定性均符合要求。结论：所建立方法专属性强,灵敏度高,精密度和准确度好,能满足进行阿昔莫司药代动力学研究的需要。