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Modifications of plant cell activities by polypeptides secreted by Eutypa lata, a vineyard fungal pathogen

机译:Eutypa lata(一种葡萄园真菌病原体)分泌的多肽对植物细胞活性的修饰

摘要

Eutypa dieback is a devastating disease induced in vineyards by the fungal pathogen Eutypa lata. The fungus colonizes the xylem tissues of trunk and cordons but is never found in the annual canes. Nevertheless, dwarfed shoots and leaf necrosis observed in diseased plants indicate that a necrotic signal can spread at a distance from the infected area. Eutypine, a small cyclic molecule, and related compounds have been postulated as the toxins inducing these symptoms. In this work, we evidence that E. lata secreted other metabolites of polypeptidic nature which induced toxic effects on canes and leaves of vines, and on leaves of other plant materials. The polypeptide fraction (PF) isolated from culture medium of mycelium induced transitory Hþ fluxes and membrane depolarization of plant cells. Complementary assays with plasma membrane vesicles (PMV) showed that Hþ-ATPase is a primary site of action as indicated by inhibition of the enzyme activity and increase of Hþ conductance of plasma membrane. The toxic effect was also obvious on respiration and photosynthesis. All these impairments led to a hindering in cell energetics and, as a consequence, to an inhibition of uptake of assimilates. Treatment with PF also triggered biological events, characteristics of elicitation as suggested by the early responses on cell membrane described above, the activation of NADPH oxidase and the activation of Phenylalanine ammonia lyase (PAL).
机译:Eutypa致死是真菌病原体Eutypa lata在葡萄园中引起的破坏性疾病。真菌定居在树干和警戒线的木质部组织中,但一年生藤茎中从未发现过。然而,在患病植物中观察到矮小的芽和叶片坏死表明坏死信号可以在距感染区域一定距离处传播。假定一个小的环状分子奥替平和相关化合物是引起这些症状的毒素。在这项工作中,我们证明了大肠埃希氏菌分泌了多肽性质的其他代谢产物,这些代谢产物对藤蔓和叶子以及其他植物材料的叶子产生了毒性作用。从菌丝体的培养基中分离出的多肽级分(PF)诱导了瞬时H +通量和植物细胞的膜去极化。用质膜囊泡(PMV)进行的补充测定表明,H + -ATPase是主要的作用位点,如酶活性的抑制和质膜H +电导率的增加所表明。对呼吸和光合作用的毒性作用也很明显。所有这些损害导致细胞能量学的障碍,并因此抑制同化物的摄取。 PF处理还触发了生物学事件,上述细胞膜上的早期反应所提示的诱发特征,NADPH氧化酶的活化和苯丙氨酸氨裂合酶(PAL)的活化。

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