Isolated or recombinant nucleic acid, expression cassette, host cell, not human, plant and transgenic seed, variant polypeptide oxox isolated or recombinant protein level modulation method of oxalate oxidase (oxox) in a plant or plant cell, method for To increase the resistance of a plant to a pathogen, a plant resistant to the pathogen.Method for identifying variants oxox with activity of oxox maintained or increased, method to generate a plant has increased resistance to a pathogen

摘要

Methods for identifying one or more amino acid substitutions in an oxalate oxidase (OXOX) variant polypeptide that confer maintained or increased OXOX activity are described herein. Methods and compositions for increasing a plant's resistance to a pathogen using the modified OXOX variant polypeptides are provided. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.