isolated, synthetic or recombinant nucleic acid, expression cassette, transformed cell, transgenic non-human animal, transgenic plant and seed, isolated polypeptide, heterodimer, homodimer, immobilized polypeptide, isolated antibody, hybridoma, food, method of producing a recombinant polypeptide , method for identifying a polypeptide, method for increasing thermotolerance, detergent composition, method for washing an object, method for stereoselectively hydrolyzing racemic mixtures, composition, method for generating a variant of a nucleic acid encoding a polypeptide with hydrolase activity , method for preparing an enzyme, lipase, cosmetic and pharmaceutical product.

摘要

Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.