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Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T

机译:南极假单胞菌PYCC 5048T将木聚糖直接转化为糖脂生物表面活性剂,甘露糖基赤藓糖醇脂质

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摘要

Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production.
机译:甘露糖基赤藓糖醇脂质(MEL)是由假单胞菌属(Pseudozyma spp。)生产的糖脂生物表面活性剂,具有越来越高的商业价值。尽管可以从d-葡萄糖和d-木糖生产MEL,但尚未报道相应的木质纤维素多糖,纤维素和木聚糖的直接转化。已评估了南极假单胞菌PYCC 5048T和蚜虫假单胞菌PYCC 5535T使用纤维素(Avicel®)和木聚糖(山毛榉木)作为碳和能源的能力,以及它们产生纤维素分解酶和半纤维素分解酶的能力,以实现整合的生物过程(CBP)用于MEL生产。在测试条件下,评估的酵母既不能在含有Avicel®的培养基中生长,也不能产生纤维素分解酶。相反,两种酵母都能在木聚糖中有效生长,但仅在南极疟原虫PYCC 5048T培养物中检测到MEL的产生。在第40天,以40 g / l的木聚糖分批培养的MEL滴度达到1.3 g / l,在以木聚糖喂养的分批补料培养物中(附加40 g / l)的MEL滴度在第4天达到高水平。在木聚糖培养物中检测到,在50°C时达到47–62 U / ml(31–32 U / mg),在生理温度(28°C)下仍显示超过10 U / ml。总的β-木糖苷酶活性主要表现为壁结合和胞外活性,分别在南极疟原虫PYCC 5048T和蚜虫PYCC 5535T培养物中分别占0.154和0.176 U / ml。目前的结果证明了假单胞菌的潜力。用于直接使用一部分木质纤维素生物质木聚糖,并在同一生物过程中将木聚糖水解酶的生产与MEL生产结合在一起。

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