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Synthesis of bacterial cellulose by Acetobacter Xylinum sp. using watermelon rind waste for biocomposite application

机译:木醋杆菌(Acetobacter Xylinum sp。)合成细菌纤维素。使用西瓜皮废料进行生物复合应用

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摘要

Cellulose was the most abundant polymer or polysaccharide that presents as the structural component of the primary cell wall of green plants but also signify for microbial extracellular polymer. The production of cellulose by microorganism such as Acetobacter xylinum sp. was most favored by researchers because the cellulose that produced was extremely pure and had a higher degree of polymerization and crystallinity than plant cellulose. The production of bacterial cellulose was expected to fulfill the high demand of cellulose in the industry. This study was focusing more on the effect of temperature and pH in the synthesis of bacterial cellulose by Acetobacter xylinum sp. using watermelon rind juice. The value of temperature and pH that being investigated was varied from 28 °C to 32 °C and from pH 4 to pH 8 respectively. The concentration for the watermelon rind juice was fixed at 7 g/L and the culture medium was incubated at fixed condition of 120 rpm. Differ from previous studies, this study use watermelon rind waste as the high potential carbon source replacing the pure carbon sources as the substrate for the synthesis of bacterial cellulose. The results data obtained shows that the optimum condition for the Acetobacter xylinum to produce the highest yield was at temperature 30 °C and pH 6 where the amount was 8.3439 g. The FT-IR analysis proves that the gelatinous membrane that produced from the experiment is cellulose. It can be shown by the appearance of absorbance peak for the C-C bonding, C-O bonding, C-OH bonding and C-O-C bonding after FT-IR analysis. In conclusion, from the data presented in this paper shows that watermelon rind waste has a high potential as the carbon source for the synthesis of bacterial cellulose and it is possible to carry out a mass production of bacterial cellulose.
机译:纤维素是最丰富的聚合物或多糖,是绿色植物初级细胞壁的结构成分,但也表示微生物细胞外聚合物。由诸如醋杆菌(Acetobacter xylinum sp。)等微生物生产纤维素。最受研究人员青睐,因为所产生的纤维素比植物纤维素具有极高的纯度,并且具有更高的聚合度和结晶度。细菌纤维素的生产有望满足工业中纤维素的高需求。这项研究更多地关注温度和pH对木醋杆菌合成细菌纤维素的影响。用西瓜皮汁。所研究的温度和pH值分别在28°C至32°C和pH 4至pH 8之间变化。西瓜皮汁液的浓度固定在7 g / L,培养基在120 rpm的固定条件下孵育。与以前的研究不同,本研究使用西瓜皮废料作为高潜力碳源,代替了纯碳源作为合成细菌纤维素的基质。获得的结果数据表明,木醋杆菌产生最高产量的最佳条件是在温度30°C和pH 6下,其量为8.3439 g。 FT-IR分析证明由实验产生的凝胶膜是纤维素。可以通过FT-IR分析后的C-C键,C-O键,C-OH键和C-O-C键的吸光度峰的出现来表示。总之,从本文提供的数据可以看出,西瓜皮废料具有很高的潜力,可以用作合成细菌纤维素的碳源,并且可以大量生产细菌纤维素。

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    Fadilah Mohamed;

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  • 年度 2010
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