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Epigallocatechingallate Inhibits Migration of Human Uveal Melanoma Cells via Downregulation of Matrix Metalloproteinase-2 Activity and ERK1/2 Pathway

机译:Epigallocatechingallate通过基质金属蛋白酶-2活性和ERK1 / 2途径的下调来抑制人体UVEAL瘤细胞的迁移

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摘要

The effects of epigallocatechingallate (EGCG) on the migration and expression of MMP-2 of uveal melanoma cells have not been reported. We studied this effect and relevant signaling pathways in a human uveal melanoma cell line (M17). MTT study found that EGCG did not affect the cell viability of M17 cells up to 100 µM. Wound-healing assay showed that EGCG significantly reduced the migration of melanoma cells in a dose-dependent manner from 20 to 100 µM. Gelatin zymography showed that secreted MMP-2 activity was dose-dependently inhibited by EGCG, whereas the MMP-2 expression at protein and mRNA levels was not affected as determined by western blot and RT-PCR analysis. EGCG significantly increased the expressions of MMP-2 endogenous inhibitors (TIMP-2 and RECK) in M17 cells. Western blot analysis of MAPK signal pathways showed that EGCG significantly decreased phosphorylated ERK1/2 levels, but not p38 and JNK levels, in melanoma cells. ERK1/2 inhibitors also reduced the migration and activity of MMP-2 in M17 cells. The present study suggested EGCG at nontoxic levels could inhibit migration of melanoma cells via downregulation of activities of secreted MMP-2 through the inhibition of the ERK1/2 phosphorylation. Therefore, EGCG may be a promising agent to be explored for the prevention of metastasis of uveal melanoma.
机译:EPigallocatechingallate(EGCG)对UVEAL黑色素瘤细胞MMP-2的迁移和表达的影响尚未报告。我们研究了人类过度的黑色素瘤细胞系(M17)中的这种效果和相关的信号通路。 MTT研究发现,EGCG不影响M17细胞的细胞活力高达100μm。伤口愈合测定表明,EGCG以20至100μM的剂量依赖性方式显着降低了黑色素瘤细胞的迁移。明胶酶谱表明,通过EGCG依赖性抑制分泌的MMP-2活性,而蛋白质和mRNA水平的MMP-2表达不受Western印迹和RT-PCR分析测定的影响。 EGCG显着增加了M17细胞中MMP-2内源抑制剂(TIMP-2和RECK)的表达。 MAPK信号途径的蛋白质印迹分析表明,在黑素瘤细胞中,EGCG显着降低了磷酸化ERK1 / 2水平,但不是P38和JNK水平。 ERK1 / 2抑制剂还降低了M17细胞中MMP-2的迁移和活性。本研究提出了在无毒水平下的EGCG可以通过抑制ERK1 / 2磷酸化通过分泌的MMP-2的活性来抑制黑素瘤细胞的迁移。因此,EGCG可以是探索预防过UVEAL黑色素瘤的转移的有望的剂。

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