Production of Polyvalent Antiserum to Ragweed Antigens in Rabbits.

摘要

Two liters of polyvalent antiserum to short ragweed pollen extract were produced in seventy young and healthy New Zealand albino rabbits. Water Soluble Ragweed (WSR) was extracted from 800grams of Ambrosia elatior pollen without defatting;final protein concentration of WSR was estimated by Biuret Method. A total of 230plus or minus 10mg of WSR,including boosting enriched preparations of Ra-3,Ra-5,were used per rabbit. Each one received no less than 12injections within a period of seven months. They were administered subcutaneously at twelve different sites each. All injecting materials were mixed 1 : 1ratio with complete Freund's adjuvant. Over 58% of the whole group responded with high titer antiserums,which were used in the final pool. There were at least ten or more precipitin bands demonstrated by Ouchterlony technique as well as immunoelectrophoretically. Specific precipitin bands against antigens E, K,and Ra-3were detected after 1 : 2dilution.