A vector comprising a nucleic acid molecule coding a rhesus D antigen is weak, bacteria or yeast comprising the same, and production processes of the antigen to test the presence of such a molecule, for the characterization of antibody or antiserum identifies Tion of VH or VL or chains of an antibody, evaluation of affinity.Greed and / or reactivity of monoclonal antibodies or polyclonal anti-D antiserum anti D or antiglobulin or antiserum of human antiglobulin, use of referu00eddo nucleic acid and kit.

摘要

Invention patent: b "nucleic acid molecules correlated with the phenotype Rhesus D weak '' d . The present invention relates to new molecules of nucleic acid coding a rhesus D antigen contributing to the weak D phenotype are characterized by one or a combination of mutations in the wrong direction or by a gene conversion involving exons 6 to 9 of the RHD, RHC gene. E.The present invention relates to vectors comprising the nucleic acid molecules of the invention, hosts transformed with such vectors, proteins encoded by said nucleic acid molecules and process for producing these polypeptides.The fact that the mutations of the wrong direction change and conversion referred to above can be directly correlated with the phenotype of weak that has a significant impact on routine test of blood samples. For example, oligonucleotides and antibodies can now be designed that usually allows the detection of phenotypes of weak in a sample.These oligonucleotides, antibodies as well as a variety of diagnostic procedures are all in the scope of the present invention antigens encoded by the new RHD. The nucleic acid molecules can be used for the characterization, standardization and quality control of anti - S. Oro anti-D monoclonal and polyclonal.Finally, the invention relates to a kit usable for testing the presence of weak D phenotypes.