Effect of Phenobarbital on Diethylnitrosamine and Dimethylnitrosamine Induced Hepatocellular Tumors in Male B6C3F1 Mice

摘要

The effect of the type of carcinogen initiator on the ability of phenobarbital to promote hepatic tumor formation in 15-day-old initiated male B6C3F1 mice was evaluated. Fifteen day old male B6C3F1 mice were divided into 6 groups of 10 mice each. Groups 1 and 2 received a single intraperitoneal (ip) injection of diethylnitrosamine (DENA) (5 micro g/gbw). Groups 3 and 4 received a single ip injection of dimethylnitrosamine (DMNA) (5 micro g/gbw). Groups 5 and 6 received a single ip injection of saline. At weaning (28 days of age), mice in groups 2, 4, and 6 received phenobarbital (500 mg/ml) in their drinking water. Mice in groups 1, 3, and 5 received deionized drinking water. Drinking water treatment continued for 24 weeks at which time mice were sampled. At sampling, mice were examined for hepatic tumors by histology. Mice in Groups 5 (no treatment) and 6 (PB only) did not exhibit hepatic tumors. Groups 2 (DENA + PB) displayed a decrease in hepatic adenomas from that of Group 1 (DENA only) confirming previous observations. Treatment with DMNA and PB (Group 4) resulted in a significant increase in both hepatic adenoma incidence and number over that of DMNA only (Group 3) treated mice. (Copyright (c) 1988 Elsevier Scientific Publishers, Ireland Ltd.)