Tissue Protective Effects of Three Supplements: Nigella Sativa Oil, Fish Oil and Sea Buckthorn Fruit, in High Caloric/High Fat Diet A comparative study

摘要

The fish oil rich in Omega-3 fatty acids, the Nigella sativa oil rich in thymoquinone and the Sea Buckthorn fruit rich in flavonoids, especially quercetin, are known for their antioxidant, anti-inflammatory and antiobesity properties. Due to these properties the study aimed to make an association between each remedy and its corresponding target tissue where the beneficial effects of the supplement is maxim. Thirty female adult Wistar rats were kept on high/caloric high fat diet (30%) and divided into five groups according to the above-mentioned supplements administered concomitantly with the diet for six weeks, as follows: Cod oil fish (EPA and DHA 0.1 g/kg), Nigella Sativa oil (0.1 g/kg), and Sea Buckthorn fruit 1g/kg. Histopathological exam of their brain, heart, and kidney was performed on their tissues and correlated with the usual plasma biological parameters and erythrocyte antioxidant enzymes. Regarding their metabolic effects, fish oil had the strongest lipid-lowering effect, and at the same time increased the HDL cholesterol significantly, the Sea buckthorn fruit corrected the high blood glucose levels, increased the erythrocyte SOD activity, while the Nigella sativa oil increased the erythrocyte GPx activity. In conclusion, by comparing the three supplements -Nigella sativa oil, fish oil and Sea Buckthorn fruit - the best neuroprotective effect was observed in the fish oil, the best renal protector was the Sea buckthorn fruit and a relative cardio protective effect was provided by Nigella sativa and Sea buckthorn supplements.