Maternal Use of Cod Oil Fish, Nigella Sativa Oil and Sea Buckthorn Fruit During Pregnancy and Lactation Period Have Protective Effects on Offspring from High Fat Diet Dams A comparative study

摘要

The aim of this study was to compare the effects of three different supplements: Cod oil fish (EPA and DHA 0.1 g/kg), Nigella Sativa oil (0.1 g/kg), and Sea Buckthorn fruit 1g/kg on pups at weaning from females with high-fat diet model of Wistar rats. Female Wistar rats (age 10 weeks) were mated and kept on high caloric/high fat diet (30%) and allocated in three groups according to the supplement associated to the fat diet. After delivery, the dams from these groups continued the treatment but with standard diet until weaning. Two more female groups were kept in parallel on the same high fat diet during gestation, but during breastfeeding one group switched on standard chow while the other continued the high fat diet. At weaning, the offspring and their dams were sacrificed and the pups' liver and pancreas were harvested. Histopathological exam was performed and tissue oxidative stress and blood biological parameters were measured. At weaning, the pups from the high fat diet dams without supplements had minor hepatopathy and minor pancreatopathy, increased tissue oxidative stress and altered metabolic profile, whereas each administered supplement proved to preserve the normal tissue aspect and also to improve biological blood and tissue oxidative stress parameters. In conclusion, maternal use of Cod oil fish, Nigella sativa oil and Sea buckthorn fruit during pregnancy and lactation period have similar protective effects on offspring from obese high fat diet dams.