Screening and Optimization of Medium Composition for Mannanase Production by Aspergillus terreus SUK-1 in Solid State Fermentation Using Statistical Experimental Methods

摘要

In this study, two statistical experimental methods including Plackett Burman design and Response Surface Methodology (RSM) were employed to optimize the medium composition for mannanase production by Aspergillus terreus SUK-1, a locally isolated fungus using Palm Kernel Cake (PKC) as the sole carbon source. A total of 19 media were screened in order to determine the optimum media for mannanase production using Plackett-Burman design. Soya bean, proteose peptone, urea and NH_4NO_3 were identified as the supplement to give positive effect on mannanase production by A. terreus SUK-1. Proteose peptone was more significant in terms of its effect on mannanase production compared to urea. The optimum of the medium composition was predicted when the concentrations of proteose peptone and urea were set at 0.5 and 1% (g g~(-1)), respectively. The optimum mannanase activity was closed to the predicted value 30.24±1.32-32.60 IU g~(-1) when the optimum medium composition was employed. Under the optimized medium (0.5% (g g~(-1)) of proteose peptone and 1% (g g~(-1)) of urea), Mannanase production was 3-fold higher than the activity obtained under original medium (9.97±1.34 IU g~(-1)).