Inhibitory Effects of Chrysanthemum zawadsaki Ethanolic Extract on Fc epsilon RI alpha Chain Expression

摘要

Human basophilic KU812F cells express a high affinity for IgE receptor, FceRI, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on Fc epsilon RI alpha chain expression. Flow cytometric analysis showed that cell surface Fc epsilon RI alpha chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for Fc epsilon RI alpha chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-Fc epsilon RI antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of Fc epsilon RI alpha chain expression and a subsequent decrease in histamine release.