Three Dimensional Model of a Complex Between FC Epsilon Receptor Alpha Chain and a FC Region of an IGE Antibody and Uses Thereof.

摘要

The present invention includes three-dimensional models of complexes between antibody receptor proteins, such as Fc-epsilon-RI-alpha. proteins, and antibodies, such as Fc-C-epsilon-3/C-epsilon-4 regions of IgE antibodies, as well as methods to produce such models. The present invention also includes muteins having increased stability and/or antibody binding activity, as well as methods to produce such muteins, preferably using information derived from three-dimensional models of the present invention. Also included are nucleic acid sequences encoding muteins of the present invention and use of those sequences to produce such muteins. Also included is the use of the model to identify compounds that inhibit the binding of an antibody receptor protein to an antibody. The present invention also includes uses of such muteins and inhibitory compounds, for example, in methods to diagnose and protect animals from allergy and other abnormal immune responses.