Optimization for the Production of Mono- and Di-acylglycerols from Corn Oil by Enzymic Glycerolysis Using Response Surface Methodology

摘要

Response surface methodology was used to optimize production conditions of monoacylglyeerol (MAG) and diaeylglycerols (DAG) from corn oil by enzymic glycerolysis. Contents of 1,3-DAG (Y_1). 1,2-DAG (Y_3), total DAG (Y_3), MAG (Y_4), and total DAG + MAG (Y_5) were obtained. Conditions were optimized using central composite design with incubation temperature (35-75°C, X_1), incubation time (1-11 hr, X_2), and amount of hexane added (0-2 mL, X_3) as three variables. Content of 1,3-DAG was maximized by20.43 area% at incubation temperature of 44.92°C, incubation time of 10.24 hr, and hexane content of 1.16 mL, whereas that of 1,2-DAG (26.78 area%) was maximized at 56.32°C, 6.95 hr, and 1.04 mL, respectively. Predicted maximum total DAG content was 45.09 area% at 53.82°C, 8.03 hr, and 1.08 mL, while production conditions of MAG (9.57 arae%) were 64.14°C, 7.00 hr, and 0.13 mL. At variables of 54.07°C, 7.98 hr, and 1.02 mL, maximum content of total DAG + MAG predicted by RSM was 53.54 area%.