Synthesis of structured lipids from corn oil and conjugated linoleic acid with immobilized lipase-catalyzed reaction

摘要

Structured lipids (SL) were synthesized by esterification of corn oil and conjugated linoleie acid (CLA) in a continuous packed-bed column reactor. The effects of flow rate, reaction temperature, and substrate molar ratios were studied. The reactionwas catalyzed by TL IM (immobilized lipase from Thermomyces lanuginosa). Results of triacylglycerol (TAG) analysis by GC showed that the incorporated CLA isomers were mainly cis9,trans11- and trans10,cis12-CLA. Slower flow rates yielded higher incorporation, and maximum incorporation of CLA was obtained with a molar ratio of 1:3 (corn oil: CLA) at a temperature of 55 deg C. The obtained SLs had iodine values ranging from 120 to 128. The SLs were composed of TAG (98 approx 99 percent), 1,2- and 1,3-diacylglycerol (0.7 approx 1.3 percent), and a small amount of monoacylglycerol.