Physicochemical Characteristics and Biological Activity of Irradiated Pectin Solution

摘要

Pectin was dissolved in HC1, citric acid, and deionized distilled water (DW, 2%, v/v) and irradiated at different irradiation doses (2.5-50 kGy) by gamma ray to investigate its physicochemical characteristics and biological activity. Viscosity of pectin solution was significantly decreased by irradiation up to 10 kGy, then remained constant thereafter. Gamma-irradiation increased monosaccharide and polysaccharide levels up to 30-40 kDa. Electron donating ability of pectin solution was highest when DWwas added and was increased by increasing irradiation dose (p < 0.05). beta-Carotene bleaching assay revealed irradiation resulted in development of antioxidantive activity in pectin solution. Growth inhibition of cancer cell lines was observed in irradiated pectin solution in dose-dependent manner, with G361 showing the highest. Results suggested irradiation of pectin solution could be effective for preparation of functional pectin oligomer.