...
首页> 外文期刊>Molecular biology of the cell >Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway
【24h】

Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway

机译:在高尔基体到ER的运输路径中形成COPI囊泡需要使用二酰基甘油

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.
机译:反式高尔基网络(TGN)到细胞表面转运必需使用二酰基甘油,但其在早期分泌途径中的功能相关性尚不清楚。尽管二酰基甘油的消耗不会影响ER向高尔基体的转运,但会导致KDEL受体向高尔基体的重新分布,表明高尔基体向ER的转运受到干扰。电子显微镜检查显示,高尔基池附近有COPI涂层的膜轮廓积聚。电子断层扫描显示,这些膜的大部分轮廓都来自被膜芽,表明膜裂变受阻。在这些条件下,ARFGAP1的高尔基体相关池减少了,但对包衣分子或膜裂变蛋白CtBP3 / BARS与高尔基体的结合没有影响。 1,2-二辛酰基-sn-甘油或二酰基甘油类似物佛波醇12,13-二丁酸酯的添加逆转了内源性二酰基甘油消耗的影响。我们的发现表明,二酰基甘油参与了蛋白质从高尔基体向内质网的逆行转运,并表明它在COPI囊泡形成的后期起着关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号