莱茵衣藻磷脂二脂酰甘油酰基转移酶3在三酰甘油合成中的功能研究

摘要

Currently, production of biodiesel by microalgae has been regarded as a promising source of renewable en-ergy. However, the understanding of oil biosynthesis mechanisms in micro-algae is limited. Phospholipid:diacylglycerol acyltransferase catalyzes phospholipid and diacylglycerol to produce triglyceride, a key reaction in triglyceride synthe-sis. In this study, we cloned a fragment of Phospholipid:diacylglycerol acyltransferase homologous gene 3 CrPDAT3 in Chlamydomonas, which was then used to construct a CrPDAT3 RNAi interference vector and transferred into Chlamy-domonas. The results showed that the growth rate of transgenic algae strain was declined. At the same time, the oil con-tent was decreased by 14.65%-45.15%, showing that the CrPDAT3 playing an important role in oil biosynthesis.%为研究磷脂二脂酰甘油酰基转移酶(PDAT)在三酰甘油合成中的功能，克隆了莱茵衣藻(Chlamydomonas reinhardtii) PDAT同源基因CrPDAT3干涉片段，通过构建CrPDAT3 RNAi 干涉载体并转化莱茵衣藻，对 CrPDAT3基因有效沉默，结果显示转基因藻株生长减缓，油脂含量下降14.65%-45.15%，说明CrPDAT3对油脂合成起到重要的作用。研究结果对于该基因应用于微藻油脂的遗传改良将起到重要作用。