Effect of photo-Fenton reaction on physicochemical parameters in white wine and its influence on ochratoxin A contents using response surface methodology

摘要

Photo-Fenton reaction is being used in different industrial processes due to their efficient degradation of toxic organic pollutants. In this study, the effect of induced photo-Fenton reaction (H2O2/Fe2+/vis) in a white wine was investigated in the presence of visible light irradiation from light-emitting diodes, at constant temperature in all the experiments. The statistical design was based on three independent factors (H2O2/Fe2+/light) and five responses titratable acidity (TA), pH, total polyphenol index (TPI), total colour differences (Delta E (ab)*) and ochratoxin A (OTA) using response surface methodology by Box-Behnken design. The total sulphur dioxide, browning index (BI) and major volatiles of the wine changes were also studied. The concentration of OTA was evaluated using enzyme-linked immunosorbent assay method. At the tested conditions, minor variations on some oenological parameters (TA and pH) were observed after photo-Fenton-controlled reaction, but other deleterious effects were observed (Delta E (ab)*, TPI and BI). The optimum 61.5 % OTA removal was achieved for irradiation dosage of 1410 lx, H2O2 and Fe2+ dosages over 2.08 and 0.085 mM L-1, respectively. This study shows that reduction of OTA may be possible using an induced photo-Fenton reaction in an artificially contaminated white wine.