Novel dextran from Pediococcus pentosaceus CRAG3 isolated from fermented cucumber with anti-cancer properties

摘要

The porous branched dextran of molecular mass 2.93 x 10~5 Da with 50% overall yield (4.5mg/ml) was produced from Pediococcus pentosaceus CRAG3 (GenBank ID: JX679020), a novel isolate from fermented cucumber. It contained glucose monomers linked through 75% a-(1,6) linkage with 25%(a-1,3) branching as displayed by spectroscopic analysis. The peak analysis showed a-(1,3) branching after every five glucose units of main chain. In vitro cytotoxicity analysis of dextran displayed anti-cancer activity against cervical cancer (HeLa) and colon cancer (HT29) cell lines opening new horizons in its pharmaceutical applications. Dextran showed enhanced growth of macrophage lines revealing its biocompatible nature.