NEW UV SPECTROPHOTOMETRIC METHODS APPLIED TO THE SIMULTANEOUS ESTIMATION OF LOSARTAN POTASSIUM AND AMLODIPINE BESYLATE IN TABLET DOSAGE FORM

摘要

Simple, sensitive and specific spectrophotometric methods were developed and validated for quantitation of Losartan potassium and Amlodipine besylate in tablet dosage form. Two new analytical methods were developed based on the simultaneous estimation of drugs in a binary mixture without previous separation. The absorption correction method is based upon determination of losartan potassium at 208 nm and amlodipine besylate at 360 nm, in methanol. In multi-component method, the binary mixture was determined by mixed standards and two sampling wavelengths of 208 nm and 360 nm. Both drugs showed linearity in a concentration range of 2-10mcg/mL for the two developed methods. The relative standard deviation value for the validation parameters was found to be 1.19 and 1.06 for absorption correction method while 0.78 and 1.56 for multi-component method, for losartan potassium and amlodipine besylate respectively in tablet formulation.