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EGFR mutation-specific antibodies in pulmonary adenocarcinoma: A comparison with DNA direct sequencing

机译:肺腺癌中EGFR突变特异性抗体:与DNA直接测序的比较

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Introduction: Epidermal growth factor receptor (EGFR) gene mutations are usually detected by direct sequencing to identify patients with advanced pulmonary adenocarcinomas as candidates for tyrosine kinase inhibitor therapy. The aim of the study was to evaluate the efficacy of EGFR mutation-specific antibodies in identifying EGFR-mutated adenocarcinomas. Materials and Methods: Thirty-three consecutive cases of pulmonary adenocarcinomas sequenced for EGFR mutations were retrieved from our files. Immunohistochemistry was performed with the rabbit monoclonal antibodies E746- A750del (6B6) and L858R (43B2). The results obtained using the 2 procedures were statistically compared by Coehn k and by calculation of sensitivity and specificity. Results: There were 21 women and 12 men, ranging in age from 48 to 78 years. All cases were lung adenocarcinomas, 23 primaries and 10 metastatic. The mutational spectrum was as follows: 12 cases mutated in exon 19 (9 with E746-A750del, 1 with homozygote L747-T751del, 1 with L747-P753del, 1 with E747-S752del), 6 in exon 21 (5 with L858R, 1 with L861Q+L862L), and 15 EGFR wild type. Immunohistochemistry detected 6/9 cases with an E746-A750del mutation (κ=0.744, sensitivity: 66.7%, specificity: 100%) and 5/5 cases with an L858R mutation (κ=1, sensitivity: 100%, specificity: 100%). Four cases showed faint and focal immunostaining and were interpreted as negative. All other cases were negative. Overall, the 2 antibodies had 61.1% sensitivity and 100% specificity for EGFR mutations.
机译:简介:表皮生长因子受体(EGFR)基因突变通常通过直接测序来检测,以鉴定患有晚期肺腺癌的患者为酪氨酸激酶抑制剂治疗的候选者。这项研究的目的是评估EGFR突变特异性抗体在鉴定EGFR突变腺癌中的功效。材料和方法:从我们的档案中检索出连续33例经EGFR突变测序的肺腺癌病例。用兔单克隆抗体E746-A750del(6B6)和L858R(43B2)进行免疫组织化学。使用Coehn k并通过计算敏感性和特异性统计比较使用这两种方法获得的结果。结果:女性21例,男性12例,年龄从48岁到78岁不等。所有病例均为肺腺癌,23例原发灶和10例转移灶。突变谱如下:第19外显子突变12例(E746-A750del 9例,纯合子L747-T751del 1突变,L747-P753del 1突变,E747-S752del 1突变),第21外显子6突变(L858R 5突变,1 L861Q + L862L)和15种EGFR野生型。免疫组织化学检测到6/9例E746-A750del突变(κ= 0.744,敏感性:66.7%,特异性:100%)和5/5例L858R突变(κ= 1,敏感性:100%,特异性:100%) )。 4例表现出淡淡的和局灶性免疫染色,并被解释为阴性。所有其他情况均为阴性。总体而言,这两种抗体对EGFR突变的敏感性为61.1%,特异性为100%。

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