Preharvest Application of Elicitors to Monastrell Grapes: Impact on Wine Polysaccharide and Oligosaccharide Composition

摘要

This work studied the effect of preharvest application in Monastrell grapes of four different elicitors [methyl jasmonate (MeJ), benzothiadiazole (BTH), chitosan from fungi (CHSf), and chitosan from seafood (CHSs)] on wine polysaccharide and oligosaccharide fractions. The polysaccharide and oligosaccharide fractions were isolated and characterized. Neutral monosaccharides were released after hydrolysis of polysaccharides and quantified by gas chromatography (GC). Sugar composition of oligosaccharides was determined after solvolysis by GC of their per-O-trimethylsilylated methyl glycoside derivatives. MeJ, BTH, CHSf, and particularly CHSs decrease the polysaccharide content in wine. The oligosaccharide concentration was also reduced after both CHS treatments. These results pointed to a lower degradation of the skin cell wall from treated grapes. We suggested that the cause would be a reinforcement of the skin cell wall as a result of the action of these elicitors. In conclusion, the application of any of these four elicitors in the clusters of the vineyard affected the complex carbohydrate composition of elaborated wine.