Multi-residue determination of 210 drugs in pork by ultra-high-performance liquid chromatography-tandem mass spectrometry

摘要

This paper presents a multi -residue analytical method for 210 drugs in pork using ultra -high-performance liquid chromatography-Q-Trap tandem mass spectrometry (UPLC MS/MS) within 20 min via positive ESI in scheduled multi-reaction monitoring (MRM) mode. The 210 drugs, belonging to 21 different chemical classes, included macrolides, sulfonamides, tetracyclines, beta-lactams, beta-agonists, aminoglycosides, antiviral drugs, glycosides, phenothiazine, protein anabolic hormones, non-steroidal anti-inflammatory drugs (NSAIDs), quinolones, antifungal drugs, corticosteroids, imidazoles, piperidines, piperazidines, insecticides, amides, alkaloids and others. A rapid and simple preparation method was applied to process the animal tissues, including solvent extraction with an acetonitrile/water mixture (80/20, v/v), defatting and clean-up processes. The recoveries ranged from 52% to 130% with relative standard deviations (RSDs) < 20% for spiked concentrations of 10, 50 and 250 mu g/kg. More than 90% of the analytes achieved low limits of quantification (LOQs) < 10 mu g/kg. The decision limit (CC alpha), detection capability (CC beta) values were in the range of 2-502 mu g/kg and 4-505 mu g/kg, respectively. This method is significant for food safety monitoring and controlling veterinary drug use. (C) 2016 Elsevier B.V. All rights reserved.