Content of Phenolic Compounds and Free Polyamines in Black Chokeberry (Aronia melanocarpa) after Application of Polyamine Biosynthesis Regulators

摘要

The total contents of anthocyanins,flavonoids,and phenolics in 60 samples of black chokeberries (Aronia melanocarpa),after treating with catabolites of polyamine biosynthesis (KPAb) and ornithine decarboxylase inhibitor,were analyzed spectrophotometrically,and quercetin and free polyamine contents were analyzed by RP-HPLC with UV detection.The average total contents of the individual substances and phenolic subgroups in control berries were as follows (mg centre dot kg~(-1)):anthocyanines,6408;flavonoids,664;phenolics,37 600;quercetin,349.KPAb decreased total contents of anthocyanines and phenolics only slightly but significantly increased the content of flavonoids.This caused an important change in the abundance of flavonoids in the pigment complex.The absolute content of quercetin was increased,but its ratio to flavonoids content was decreased.Ornithine decarboxylase inhibitor had a markedly different effect as it significantly increased total content of anthocyanins and total phenolics,inhibited the total content of free polyamines,and stimulated the processes of saccharides transformation to phenolic pigments.