Towards the biotechnological isomerization of branched sugars:D-tagatose-3-epimerase equilibrates both enantiomers of 4-C-methyl-ribulose with both enantiomers of 4-C-methyl-xylulose

摘要

Microbial oxidation of 2-C-methyl-D-ribitol and 2-C-methyl-D-arabinitol by Gluconobacter thailandicus NBRC 3254 produces 4-C-methyl-L-ribulose and 4-C-methyl-D-ribulose,respectively.Further,4-C-methyl-L-ribulose and 4-C-methyl-D-ribulose were equilibrated by D-tagatose-3-epimerase (DTE) with 4-C-methyl-L-xylulose and 4-C-methyl-D-xylulose,respectively.These transformations demonstrate that polyol dehydrogenase and DTE act on branched synthetic sugars.The green preparation of all of the stereoisomers of 4-C-methyl pentuloses illustrates the ability of biotechnology to generate novel branched monosaccharides.