Identification of chloroplast DNA variations by PCR-RFLP analysis in Dendranthema

摘要

Variations in chloroplast DNA of Dendranthema species were studied by PCR-RFLP analysis to seel, the maternal origin of the cultivated chrysanthemum, D. grandiflorum. Ten genes, atpH, matK, petA, petB, psaA, rbcL, rpoB, rpoC, trnK and 16S in the chloroplast DNA of 12 Japanese wild species and 1 cultivar were amplified by PCR. The amplified DNAs that were digested with each of 32 restriction endonucleases revealed 13 site changes among the 13 species in the following 9 gene plus restriction endonuclease combinations: petA-AvaII, petA-HaeIII, petA-Mboll, petA-NdeII, rpoC-EcoRV, trnK-DraI, trnK-HinfI, trnK-MboII and trnK-ScrFI. No Japanese wild species showed the same PCR-RFLP pattern of chloroplast DNA as D. grandiflorum.