首页> 外文期刊>Journal of Neuroscience Research >Flavoprotein autofluorescence imaging in the cerebellar cortex in vivo.
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Flavoprotein autofluorescence imaging in the cerebellar cortex in vivo.

机译:体内小脑皮质的黄素自体荧光成像。

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摘要

Autofluorescence optical imaging is rapidly becoming a widely used tool for mapping activity in the central nervous system function in vivo and investigating the coupling among neurons, glia, and metabolism. This paper provides a brief review of autofluorescence and of our recent work using flavoprotein imaging in the cerebellar cortex. Stimulation of the parallel fibers evokes an intrinsic fluorescence signal that is tightly coupled to neuronal activation and primarily generated postsynaptically. The signal originates from mitochondrial flavoproteins. The signal is biphasic, with the initial increase in fluorescence (light phase) resulting from the oxidation of flavoproteins and the subsequent decrease (dark phase) from the reduction of flavoproteins. The light phase is primarily neuronal, and the dark phase is primarily glial. Exploiting the spatial properties of molecular layer inhibition in the cerebellar cortex, we show that flavoprotein autofluorescence can monitor both excitatory and inhibitory activity in the cerebellar cortex. Furthermore, flavoprotein autofluorescence has revealed that molecular layer inhibition is organized into parasagittal domains that differentially modulate the spatial pattern of cerebellar cortical activity. The reduction in flavoprotein autofluorescence occurring in the inhibitory bands most likely reflects a decrease in intracellular Ca(2+) in the neurons inhibited by the molecular layer interneurons. Therefore, flavoprotein autofluorescence imaging is providing new insights into cerebellar cortical function and neurometabolic coupling.
机译:自体荧光光学成像正迅速成为一种广泛使用的工具,用于在体内绘制中枢神经系统功能的活动并研究神经元,神经胶质和新陈代谢之间的耦合。本文简要回顾了自发荧光以及我们最近在小脑皮层中使用黄素蛋白成像的工作。平行纤维的刺激唤起了固有的荧光信号,该信号与神经元的激活紧密耦合并主要在突触后产生。信号来自线粒体黄素蛋白。信号是双相的,荧光的最初增加(亮相)是由于黄素蛋白的氧化所致,随后是降低的(暗相)是黄素蛋白的还原所致。亮相主要是神经元,暗相主要是神经胶质。利用小脑皮层中分子层抑制的空间特性,我们发现黄素蛋白自发荧光可以监测小脑皮层中的兴奋性和抑制性活动。此外,黄素蛋白自发荧光显示分子层抑制被组织成矢状旁区域,该域有差别地调节小脑皮质活动的空间模式。抑制带中发生的黄素蛋白自发荧光的减少最有可能反映了分子层间神经元所抑制的神经元中细胞内Ca(2+)的减少。因此,黄素蛋白自发荧光成像为小脑皮质功能和神经代谢耦合提供了新的见解。

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