LC-DAD-ESI-MS-MS Separation and Chemical Characterization of the Inflammatory Fraction of the Roots of Euphorbia kansui

摘要

In China the roots of Euphorbia kansui T.N. Liou ex T.P. Wang, known as 'kansui', have been used for centuries as a herbal remedy for edema, ascites, and asthma. Kansui, has inflammatory and tumor-promoting toxicity, and other side-effects, however, which have seriously restricted its clinical application. In the work discussed in this paper a simple and rapid LC-DAD-ESI-MS-MS method has been established for separation and characterization of the main compounds in the toxic fraction of E. kansui roots. Twelve diterpene derivatives were identified in the inflammatory fraction of kansui: kansuinine C, kansuinine B, kansuinine A, kansuinine D, 5-O-benzoyl-20-deoxyingenol, kansuinine E, kansuiphorin C, 3-O-benzoyl-20-deoxyingenol, 3-O-(2'E,4'Z-decadienoyl)-5-O-acetylingenol, 3-O-(2'E,4'Z-decadienoyl)-20-deoxyingenol, 20-O-(2'E, 4'E-decadienoyl)ingenol, and 3-O-(2'E,4'Z-decadienoyl)-20-O-acetylingenol. The inflammatory fraction was separated on a C-18 reversed-phase column with a mobile phase gradient. The proposed method is a scientific and technical platform enabling the herbal medicine industry to perform quality control and ensure the safety of preparations that contain this class of poisonous diterpenoids.