Aflatoxin B_1 Degradation by Flavobacterium aurantiacum in the Presence of Reducing Conditions and Seryl and Sulfhydryl Group Inhibitors

摘要

This study was undertaken to determine the effects of reducing conditions (l-cysteine) and seryl (phenylmethylsulfonyl fluoride) and sulfhydryl (divalent cadmium) group inhibitors on aflatoxin B_1 (AFB1) degradation by Flavobacterium auran- tiacum. High-performance liquid chromatography was used to determine AFB1 concentrations in 72-h cultures of F. auran- tiacum. The addition of 0.1, 1, or 10 mM l-cysteine did not have any significant effect on AFB1 degradation by these cultures after incubation for 4, 24, or 48 h (P > 0.05). The addition of 0.1 mM phenylmethylsulfonyl fluoride did not significantly decrease AFB1 degradation (P > 0.05), but 1 mM phenylmethylsulfonyl fluoride significantly decreased AFB1 degradation after 4, 24, and 48 h of incubation (P ≤0.05).