Rapid Analysis of 2-Alkylcyclobutanones in Irradiated Meats, Cheese and Salmon by Direct Solvent Extraction Followed by GPC

摘要

2-Alkylcyclobutanones (2-ACBs) are recognized as a marker of irradiation in lipid-containing food products. Here, a rapid method for the analysis of 2-dodecylcyclobutanone (2-DCB) and 2-tetra-decylcyclobutanone (2-TCB) in irradiated food products using direct solvent extraction (DSE) was developed, and the extraction efficiency was compared to that of the Soxhlet extraction method (EN 1785), which is the official method for this analysis. Briefly, 2-ACBs were extracted either by using a Soxhlet apparatus or by DSE with n-hexane. The lipid extract was purified by GPC followed by a silica gel cartridge column. Finally, 2-DCB and 2-TCB were measured using GC-MS/MS. The extraction efficiency of 2-ACBs by the DSE method was similar to that of the Soxhlet extraction method. The trueness of 2-DCB and 2-TCB spiked at 50 ng/g in lipid extracts of beef, pork, chicken, cheese and salmon with the proposed method were 76.6％ to 91.6％ and 81.3％ to 109.0％, respectively. The limits of detection for 2-DCB and 2-TCB in lipid extracts were 15 and 20 ng/g, respectively.%2-アルキルブタノン類(2-ACBs)は含脂質食品の放射線照射により生成することが知られている.今回,直接溶媒抽出による放射線照射食品からの2-ドデシルシクロブタノン(2-DCB)と2-テトラデシルシクロブタノン(2-TCB)の抽出法を開発し，EUの標準分析法(EN1785)であるソックスレー抽出との抽出効率を比較した.放射線照射した試料はへキサンでソックスレーもしくは直接抽出した.抽出脂質はGPCおよびシリカゲルミニカラムにより精製し,2-DCBと2-TCBをGC-MS/MSで測定した.直接抽出法はソックスレー抽出と同等の抽出効率が得られた.牛肉，豚肉,鶏肉，チーズおよびサーモンの脂質抽出物に50ng/gの濃度となるように2-DCBおよび2-TCBを添加したときの本法の真度は，それぞれ76.6〜91.6％および81.3〜109.0％であった.2-DCBおよび2-TCBの検出限界はそれぞれ15ng/gおよび20ng/gであった.