汎用マトリッタス添加標準溶液を活用した野菜類および果実類中の残留農薬一斉分析法の妥当性評価

摘要

食品中に残留する農薬等に関する試験法について，平成25年12月13日までに「食品中に残留する農薬等に関する試験法の妥当性評価ガイドライン」(以下，ガイドライン)に則り評価を行うことが必要となった.筆者らは,QuEChERS(Quick,Easy,Cheep,Effective,Rugged and Safety)法を参考に迅速な残留農薬一斉分析法を独自に開発し，ばれいしよ，ほうれんそうおよびりんご等の代表的な野菜および果実類についてLC-MS/MSによる評価を行い既報で報告した.今回はLC-MS/MSの測定では十分に感度が得られない農薬のうち，GC-MS/MSの測定に適した農薬を対象に妥当性評価を行った.%Quantitative methods using the matrix-matched standard solutions approach are widely used for multi-residue pesticide determination by GC-MS/MS to deal with the issue of matrix effects. However, preparing matrix-matched standard solutions in analyses of many kinds of samples is very time-consuming. In order to solve this problem, a method that employs general matrix standard solutions has been developed using polyethylene glycol (PEG), extract of vegetables-fruit juice (VFJm) and triphenyl phosphate (named the PEG-VFJm method). Here, a validation study for 168 pesticides was performed on three kinds of samples [potato, spinach and apple] at concentrations of 0.010 and 0.050 μg/g. In these three commodities,144 to 158 pesticides satisfied the required criteria using the matrix-matched method and 129 to 149 pesticides satisfied the same criteria using the PEG-VFJm method. Our results suggest that application of general matrix standard solutions would enable rapid and effective analyses of pesticides.