Convenient Partial Purification Of Polyphenol Oxidase From Apple Skin By Cationic Reversed Micellar Extraction

摘要

Polyphenol oxidase (PPO) was selectively extracted from reconstituted freeze-dried apple skin using reverse micelles formed by a cationic surfactant,dodecyl trimethyl ammonium bromide (DTAB).An optimum forward extraction was achieved with sodium phosphate buffer (pH 6,100 mM,no added KCl) and an organic phase (isooctane:hexanol at a ratio of 5:1) containing lOOmM DTAB.The solubilised PPO was efficiently recovered by a stripping solution (pH 6,1 M KCl) containing 10% ethanol.Under the optimised conditions,the purification fold and recovered activity of PPO were 12.6% and 71%,respectively.This purification fold and recovery were maintained when the extraction volume increased from 10-200 ml.Overall,reversed micellar extraction can be used as an efficient first step for the purification of PPO from apple skin.