Comparison between some different methods for determination of Aflatoxin M1 in milk and some diary products

摘要

Seven TLC methods for evaluate the incidence of Aflatoxin M1 in milk and dairy products were used, Pons et al . (1973); Fukayama et al . (1980); Stubblefield (1979); Official Method of Analysis (1995); Van Egmond and Stubblefield (1981); Official Method of Analysis (1984) and Official Method of Analysis (1990) , to detect the sensitive method among them for determination of Aflatoxin M1 (AFM1). Results show that, the Official Method of Analysis, 1995 (AOAC, 1995) was the sensitive method for determination of AFM1 among the all tested methods, where it gives the highest recovery percentage for liquid milk, Cheese, and Powdered milk, 106.2%, 95.99% and 104.4% respectively. While Stubblefield, (1979) method gave about 75% AFM1 recovery for Yogurt, but when we made a slight modification on it gave 92% AFM1 recovery