Functional constraints on SoxE proteins in neural crest development: The importance of differential expression for evolution of protein activity

Eric M. Lee; Tian Yuan; Reyna D. Ballim; Kristy Nguyen; Robert N. Kelsh; Daniel M. Medeiros; David W. McCauley

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Functional constraints on SoxE proteins in neural crest development: The importance of differential expression for evolution of protein activity

机译：SoxE蛋白质在神经c发育中的功能限制：差异表达对蛋白质活性进化的重要性

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VertebrateSoxEgenes(emSox8/em,em9/em,andem10/em)arekeyregulatorsofneuralcrestcell(NCC)development.ThesegenesarosebyduplicationfromasingleSoxEgeneinthevertebrateancestor.AlthoughSoxEparalogsarecoexpressedearlyinNCCdevelopment,later,emSox9/emisrestrictedtoskeletogeniclineagesinthehead,andemSox10/emtonon-skeletogenicNCCinthetrunkandhead.Whenthissubfunctionalizationevolvedanditspossibleroleintheevolutionoftheneuralcrestareunknown.SealampreysarebasalvertebratesthatalsopossessthreeSoxEgenes,whileonlyasingleSoxEispresentinthecephalochordateamphioxus.InordertoaddressthefunctionaldivergenceofSoxEgenes,andtodetermineifdifferencesintheirbiochemicalfunctionsmaybelinkedtochangesinneuralcrestdevelopmentalpotential,weexaminedtheabilityoflampreyandamphioxusSoxEgenestoregulatedifferentiationofNCCderivativesinzebrafishemcolourless/em(emcls/em)mutantslackingexpressionofemsox10/em.Ourfindingssuggestthattheproto-vertebrateemSoxE/emgenepossessedbothmelanogenicandneurogeniccapabilitiespriortoSoxEgeneduplication.Followingtheagnathan-gnathostomesplit,lampreyemSoxE1/emandemSoxE3/emlargelylosttheirmelanogenicand/orentericneurogenicproperties,whilegnathostomeSoxEparalogshaveretainedfunctionalconservation.WepositthatthisdifferenceinproteinsubfunctionalizationisadirectconsequenceoftheindependentregulationofSoxEparalogexpressionbetweenthetwolineages.Specifically,weproposethattheoverlappingexpressionofgnathostomeSoxEparalogsinearlyneuralcrestlargelyconstrainedthefunctionofgnathostomeSoxEproteins.Incontrast,thelargelynon-overlappingexpressionoflampreySoxEparalogsallowedthemtospecializewithregardtotheirDNA-bindingand/orproteininteractionproperties.RestrictionofdevelopmentalpotentialamongcranialandtrunkneuralcrestinlampreysmayberelatedtoconstraintsonSoxEactivityamongduplicates,butsuchspecializationdoesnotappeartohaveoccurredingnathostomes.ThishighlightsanimportantdifferenceintheevolutionofSoxEactivitybetweenthesetwodivergentvertebratelineagesandprovidesinsightsforunderstandinghowcellfaterestrictionindifferentNCCpopulationsmaybedependentonsubfunctionalizationamongSoxEduplicates./p/div

机译：VertebrateSoxEgenes（ Sox8 ， 9 和 10 ）是神经（细胞（NCC）发展的关键调节器。 isrestrictedtoskeletogeniclineagesinthehead和 SOX10 的TONON-skeletogenicNCCinthetrunkandhead.Whenthissubfunctionalizationevolvedanditspossibleroleintheevolutionoftheneuralcrestareunknown.SealampreysarebasalvertebratesthatalsopossessthreeSoxEgenes，whileonlyasingleSoxEispresentinthecephalochordateamphioxus.InordertoaddressthefunctionaldivergenceofSoxEgenes，andtodetermineifdifferencesintheirbiochemicalfunctionsmaybelinkedtochangesinneuralcrestdevelopmentalpotential，weexaminedtheabilityoflampreyandamphioxusSoxEgenestoregulatedifferentiationofNCCderivativesinzebrafish <位置>无色的（<在>顺的）mutantslackingexpressionof <位置> SOX10 < / em>。我们的发现建议，原始脊椎动物 SoxE 基因可能具有黑素生成和中性生成ccapabilitiespriortoSoxEgeneduplication.Followingtheagnathan-gnathostomesplit，八目鳗<位置> SoxE1 和 SoxE3 的largelylosttheirmelanogenicand / orentericneurogenicproperties，whilegnathostomeSoxEparalogshaveretainedfunctionalconservation.WepositthatthisdifferenceinproteinsubfunctionalizationisadirectconsequenceoftheindependentregulationofSoxEparalogexpressionbetweenthetwolineages.Specifically，weproposethattheoverlappingexpressionofgnathostomeSoxEparalogsinearlyneuralcrestlargelyconstrainedthefunctionofgnathostomeSoxEproteins.Incontrast，thelargelynon-overlappingexpressionoflampreySoxEparalogsallowedthemtospecializewithregardtotheirDNA-bindingand / orproteininteractionproperties.RestrictionofdevelopmentalpotentialamongcranialandtrunkneuralcrestinlampreysmayberelatedtoconstraintsonSoxEactivityamongduplicates，butsuchspecializationdoesnotappeartohaveoccurredingnathostomes。这两个不同的脊椎动物世系之间的Sox活性演变有着重要的区别，并提供了ghts了解不同NCC种群的细胞命运限制可能取决于Sox重复项中的亚功能化。 展开▼

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《Developmental biology》 |2016年第1期|共页

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Eric M. Lee; Tian Yuan; Reyna D. Ballim; Kristy Nguyen; Robert N. Kelsh; Daniel M. Medeiros; David W. McCauley;
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中图分类生物科学;

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专利

1. Functional constraints on SoxE proteins in neural crest development: The importance of differential expression for evolution of protein activity [J] . Lee Eric M., Yuan Tian, Ballim Reyna D., Developmental biology . 2016,第1期

机译：SoxE蛋白质在神经c发育中的功能限制：差异表达对蛋白质活性进化的重要性

2. Differential requirement of bone morphogenetic protein receptors Ia (ALK3) and Ib (ALK6) in early embryonic patterning and neural crest development [J] . Carolin Schille, Jens Heller, Alexandra Schambony BMC Developmental Biology . 2016,第1期

机译：早期胚胎形成和神经c发育中骨形态发生蛋白受体Ia（ALK3）和Ib（ALK6）的差异需求

3. Differential expression of the actin-binding proteins, alpha-actinin-2 and -3, in different species: implications for the evolution of functional redundancy. [J] . Mills M, Yang N, Weinberger R, Human Molecular Genetics . 2001,第13期

机译：肌动蛋白结合蛋白α-actinin-2和-3在不同物种中的差异表达：对功能冗余进化的影响。

4. PHOSPHOLIPASE D ACTIVITIES AND PHOSPHATIDYLCHOLINE TURNOVER ARE DIFFERENTIALLY RELATED TO EXPRESSION OF PROTEIN KINASE C ISOFORMS AND MARCKS IN CONTROL AND TRANSFECTED NEURAL CELLS [C] . Harold W. Cook, Stephen C. Van Iderstine, Sherry C. Morash, International Washington Spring Symposium . 1996

机译：磷脂酶D活性和磷脂酰胆碱转口与对照和转染神经细胞的蛋白激酶C同种型和马克斯的表达差异差异。

5. The Regulation of SoxE Transcription Factors in Neural Crest Development. [D] . Lee, Pei-Chih. 2012

机译：SoxE转录因子在神经rest发育中的调节。

6. Differential Functional Constraints on the Evolution of Postsynaptic Density Proteins in Neocortical Laminae [O] . Guang-Zhong Wang, Genevieve Konopka 2009

机译：在新皮层中突触后密度蛋白的进化差异功能约束。

7. Functional constraints on SoxE proteins in neural crest development: the importance of differential expression for evolution of protein activity [O] . Lee Eric M., Yuan Tian, Ballim Reyna D., 2016

机译：SoxE蛋白质在神经development发育中的功能限制：差异表达对于蛋白质活性进化的重要性

1. 股骨头缺血性坏死组织差异表达蛋白质的鉴定和功能蛋白质组学初步研究 [J] . 张雷 ,杨国敬 ,王珺n . 中华关节外科杂志（电子版） . 2011,第004期

2. 牛蛙蝌蚪变态发育中的蛋白质差异表达 [J] . 范丽华 ,虞鹏程 ,王三英 . 南昌大学学报（理科版） . 2007,第005期

3. 胆囊良恶性组织中蛋白质组差异表达及膜联蛋白A3的功能 [J] . 谈燚 ,孟海萍 ,崔培元 . 中华实验外科杂志 . 2010,第007期

4. 蛋白质小泛素样修饰体化修饰对神经发育和功能的影响 [J] . 肖宪玲 ,郑军 ,刘晓智 . 发育医学电子杂志 . 2018,第002期

5. 双孢蘑菇子实体发育后期差异表达蛋白质分析 [J] . 陈美元 ,廖剑华 ,李洪荣 . 菌物学报 . 2013,第005期

6. 疏水性在蛋白质结构与功能教学中的重要性 [C] . 达朝山 . 首届全国生物化学与分子生物学教学研讨会 . 2007

7. 基于基因表达和蛋白质相互作用数据集成的蛋白质进化、功能重要性和动态模块化组织的研究 [A] . 庞开放 . 2011

1. 弓形虫感染小鼠脑组织差异表达蛋白质、与大脑发育调节蛋白相互作用的蛋白质及其应用 [P] . 中国专利： CN106810602A . 2017-06-09

2. 具有催泪成分合成酶活性的蛋白质或多肽、编码该蛋白质或多肽的 DNA、利用该 DNA制造具有催泪成分合成酶活性的蛋白质或多肽的制造方法以及具有抑制该蛋白质或多肽的mRNA翻译的功能的核酸分子 [P] . 中国专利： CN1639337A . 2005-07-13

3. selection method. evolution and expression of an antibody fragment or antibody, methods of evolution and expression of an antibody and a protein, method of evolution and manufacture of a protein, method of evolution for enhanced expression and manufacture of a protein, method for identifying and produce a protein, and method of evolution of a protein. [P] . 外国专利： BR112012001171A2 . 2017-06-13

机译：选择方法。抗体片段或抗体的进化和表达，抗体和蛋白质的进化和表达方法，蛋白质的进化和制备方法，增强的蛋白质表达和制备的进化方法，鉴定和产生蛋白质的方法以及蛋白质进化的方法。

4. gene expression construct, gene expression system, variance of a gene expression system, process for increasing expression or enhancing the translational activity of a sequence derived from a rna-2 genome segment of a bipartite virus, methods to express a protein of interest in a host organism, to improve translation of a heterologous protein of interest from a gene or open reading frame, to express a protein gene expression construct, gene expression system, variation of a system Gene Expression, A Process for Increasing Expression or Improving the Translational Activity of a Sequence Derived from a Rna-2 Genome Segment of a Bipartite Virus, Methods for Expressing a Protein of Interest in a Host Organism, to Improve Translation of a protein of heterologous interest of an open reading frame or gene to express a protein in a plant and plant to generate a protein of interest, and host organism [P] . 外国专利： BRPI0906509A2 . 2015-12-01

机译：基因表达构建体，基因表达系统，基因表达系统的变异，增加表达或增强源自二分病毒的rna-2基因组片段的序列的翻译活性的过程，在宿主中表达目的蛋白的方法生物体，以改善来自基因或开放阅读框的目的异源蛋白质的翻译，以表达蛋白质基因表达构建体，基因表达系统，系统的变体基因表达，增加表达或改善其翻译活性的过程源自二分病毒的Rna-2基因组片段的序列，用于在宿主生物中表达目标蛋白质的方法，以改善开放阅读框或基因的异源目标蛋白质在植物中表达该蛋白质的方法，以及植物产生目的蛋白和宿主生物

5. Nucleic acid sequence, amino acid sequence, replicative cloning vector, isolated host cell expression vector, methods for testing a substance as a therapeutic agent for bone modulation in a host to identify a molecule and a candidate protein involved in bone modulation, to test for hbm activity, for pharmaceutical development to treat bone development disorders, to treat a bone development disorder in an animal, to alter bone development in a host, to treat osteoporosis, to evaluate diagnosis for a genetic predisposition to a bone development disorder, to identify a genetic predisposition to bone development disorders, to express hbm protein in bone tissue, to amplify a nucleotide polymorphism, to identify a regulatory element of an hbm gene, and to modulate bone density and in one patient, diagnostic assay for bone development disorders, bacterial artificial chromosome, isolated nucleic acid segment, nucleic acid, and polypeptide. [P] . 外国专利： BR0017197A . 2003-01-14

机译：核酸序列，氨基酸序列，复制性克隆载体，分离的宿主细胞表达载体，在宿主中测试作为骨调节治疗剂的物质的方法以鉴定参与骨调节的分子和候选蛋白以测试hbm活性，用于药物开发以治疗骨骼发育异常，治疗动物骨骼发育异常，改变宿主的骨骼发育，治疗骨质疏松症，评估对骨骼发育异常的遗传易感性诊断，鉴定遗传易患骨发育障碍，在骨组织中表达hbm蛋白，扩增核苷酸多态性，鉴定hbm基因的调节元件并调节骨密度，并且在一名患者中进行骨发育障碍的诊断测定，细菌人工染色体，分离的核酸片段，核酸和多肽。

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Functional constraints on SoxE proteins in neural crest development: The importance of differential expression for evolution of protein activity

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