selection method. evolution and expression of an antibody fragment or antibody, methods of evolution and expression of an antibody and a protein, method of evolution and manufacture of a protein, method of evolution for enhanced expression and manufacture of a protein, method for identifying and produce a protein, and method of evolution of a protein.

摘要

The present disclosure provides methods of integrating therapeutic protein and antibody generation and/or selection, evolution and expression in a eukaryotic host for manufacturing in a single system. Therapeutic proteins, including antibodies, are generated, optimized and manufactured in the same eukaryotic host system. The disclosed system of Comprehensive Integrated Antibody Optimization (CIAO!„¢) allows for simultaneous evolution of protein performance and expression optimization.