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首页> 外文期刊>Developmental biology >Differentiation of presumptive primordial germ cell (pPGC)-like cells in explants into PGCs in experimental tadpoles
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Differentiation of presumptive primordial germ cell (pPGC)-like cells in explants into PGCs in experimental tadpoles

机译:实验性t中外植体中推定的原始生殖细胞(pPGC)样细胞向PGC的分化

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摘要

Asingleblastomerecontainingthea€?germplasma€?of32-cellstageXenopusembryoswasculturedwith[3H]thymidineuntilthecontrolembryosdevelopedtotheneurulastage.Theexplants,showingasphericalmassinwhichthenucleiofallcellswerelabeled,wereimplantedintotheprospectiveplaceofpresumptiveprimordialgermcells(pPGCs)intheendodermalcellmassofunlabeledhostembryosoftheneurulastage.LabeledPGCsaswellasunlabeled,hostPGCswerefoundinthegenitalridgesofexperimentaltadpoles.Thisindicatesthattheprecursorofgermcells,correspondingtopPGCsinnormalembryosoftheneurulastage,intheexplantsmigratedtogenitalridgesjustattherightmomenttobecomePGCs,andsuggeststhatthedevelopmentalprocessprogressednormally,evenintheexplants,asfarasthedifferentiationofpPGCsisconcerned.
机译:Asingleblastomerecontainingthea€?germplasma€?of32-cellstageXenopusembryoswasculturedwith [3H] thymidineuntilthecontrolembryosdevelopedtotheneurulastage.Theexplants,showingasphericalmassinwhichthenucleiofallcellswerelabeled,wereimplantedintotheprospectiveplaceofpresumptiveprimordialgermcells(pPGCs)intheendodermalcellmassofunlabeledhostembryosoftheneurulastage.LabeledPGCsaswellasunlabeled,hostPGCswerefoundinthegenitalridgesofexperimentaltadpoles.Thisindicatesthattheprecursorofgermcells,correspondingtopPGCsinnormalembryosoftheneurulastage,intheexplantsmigratedtogenitalridgesjustattherightmomenttobecomePGCs,andsuggeststhatthedevelopmentalprocessprogressednormally,evenintheexplants,asfarasthedifferentiationofpPGCsisconcerned。

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