MAINTENANCE-AND-AMPLIFICATION METHOD AND DIFFERENTIATION INDUCTION METHOD FOR PRIMORDIAL GERM CELLS/PRIMORDIAL GERM CELL—LIKE CELLS

摘要

The present invention provides: a maintenance-and-amplification method for PGC/PGCLC, the method involving culturing PGC/PGCLC in the presence of a phosphodiesterase 4 (PDE 4) inhibitor and/or cyclosporin A, and preferably in the presence of forskolin; and a method for inducing oocytes from PGC/PGCLC, the method involving culturing PGC/PGCLC in the presence of bone morphogenetic protein (BMP) and retinoic acid (RA).