cell line primordial germ cells (PGCs) bird and method for long-term culture thereof.

摘要

A culturing method which provides maintenance of primordial germ cells of birds for periods of at least 14 days in tissue culture comprising the following steps: (i) isolating a pure population of primordial germ cells from a desired bird; and (ii) culturing said pure, isolated population of primordial germ cells (PGCs) in a culture medium containing at least the following growth factors contained in amounts sufficient to maintain said PGCs for at least fourteen days in tissue culture: (1) leukemia inhibitory factor (LIF), (2) growth factor basic fibroblast (bFGF), (3) cell factor axis (SCF) and (4) factor, insulin-like growth factor (IGF), for a extended period.